Does CRISPR-Cas9 Have Off-Target Effects?

A genome-wide profiling method shows that CRISPR/Cas9 gene editing has off-target effects at a frequency of less than 0.1 percent.

AsianScientist (Feb. 23, 2015) – A team of researchers from Korea’s Institute for Basic Science (IBS) has successfully confirmed that CRISPR-Cas9 has accurate on-target effects in human cells, through joint research with the Seoul National University College of Medicine and ToolGen, Inc. Their findings have been published in Nature Methods.

There has been great interest in CRISPR-Cas9 as a tool to develop anticancer cell therapies or to correct genetic defects that cause hereditary in stem and somatic cells. However, since there has been no reliable and sensitive method to measure the accuracy of CRISPR-Cas9 genome-wide, its safety has remained in question. Consequently, it has been difficult to eliminate the possibility that CRISPR-Cas9 may induce mutations in off-target sequences that are similar to on-target sequences. Off-target mutations in tumor suppressor genes, for example, can cause cancer.

The researchers have developed a technique termed Digenome-seq to locate both on-target and off-target sequences that can be mutated by CRISPR-Cas9 via genome sequencing. They digested human genomic DNA using Cas9 nucleases in a test tube, which was then subjected by whole genome sequencing. This in vitro digest yielded a unique pattern at both on-target and off-target sequences that can be computationally identified.

Furthermore, by adding guanine nucleotides at the end of single guided RNA that composes CRISPR-Cas9, they created a highly-developed programmable nuclease, which has no measurable off-target effects in the human genome.

Dr. Kim Jin-Soo, the director of the Center for Genome Engineering at IBS, as well as the professor of the Department of Chemistry at Seoul National University says, “If CRISPR-Cas9 truncates off-target DNA sequences, it might induce unwanted mutations. Since we have succeeded in confirming the accuracy of CRISPR-Cas9, we anticipate that there will be a great progress in the development of gene or cell therapies.”

The article can be found at: Kim et al. (2015) Digenome-seq: Genome-wide Profiling of CRISPR-Cas9 Off-Target Effects in Human Cells.


Source: Institute for Basic Science.
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