Small Non-Coding RNAs Can Cross The Placenta, Scientists Say

Researchers claim that small exogenous RNAs from plants eaten by a mother can cross the placenta and affect fetal development.

AsianScientist (May 21, 2015) – Small non-coding ribonucleic acids (RNAs) in maternal food can transfer through placenta to regulate fetal gene expression, according to a study done by researchers at Nanjing University, which has been published in Protein & Cell.

“According to what I know, this is the first study to examine the transplacental transmission of small-noncoding RNAs,” said Professor Hu Yali, from the department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital.

“This finding also proposes a brand new potential strategy to treat fetal diseases in utero. Given that artificial synthetic siRNAs can transfer through placenta, we can try to use gene therapy to treat fetal disease by maternal administration,” Hu said.

MicroRNAs (miRNA) are a class of noncoding RNAs with lengths of approximately 22 nucleotides that bind to target messenger RNAs to inhibit protein translation. In previous studies, the same group has found that plant miRNAs can enter into the host blood and tissues via the route of food-intake. The food-derived exogenous miRNAs are absorbed, packaged into microvesical (MV) and then secreted into circulation by cells of animal gastrointestinal tract. More importantly, once inside the host, the food-derived exogenous miRNAs can regulate host physiology by regulating host ‘target’ genes in the cross-kingdom manner.

Here, they report another surprising finding that exogenous plant miRNAs and artificial synthetic small influence RNAs (siRNAs) can transfer through the placenta and directly regulate fetus gene expression. Firstly, exogenous plant miRNAs was detected in human umbilical cord blood, amniotic fluid as well as animal foetuses with certain level.

When pregnant mice were administrated honeysuckle soup (the exogenous plant microRNAs are physiological concentration in food), the plant MIR2911 was detected in fetus liver with significant level. Finally, feeding pregnant mice with synthetic alpha-fetoprotein (AFP, only expressed in foetus liver) siRNA decreased significantly AFP mRNA and protein levels. They have further demonstrated that MV-driven small RNAs are able to pass through placenta.

This is the first time that it has been demonstrated that small RNAs can pass through mammalian placenta and directly regulate fetus gene and may consequently also influence fetus development. It is well known that the placenta is a vital organ on which the survival and growth of the fetus are critically dependent on. It forms the interface between the maternal and fetal environments, facilitating the exchange of gases, nutrients and waste products between the mother and baby and also acts as a barrier against the maternal immune system.

“The classical concept is that nucleic acid is not able to pass through the mammalian placenta, while we have also demonstrated that MV-driven small RNAs are able to pass through placenta,” Dr. Zhang Yujing said.

This discovery is important to further understand the function of placenta and maternal and fetal environments.

Exogenous small RNAs in food not only affect pregnant female, but influence fetus development as well. The dietary patterns of the mother will influence the fetus or even determine the postnatal health status. Dietary bias or other unhealthy dietary habits would also affect the fetus health by disrupting the balance of transplacental miRNAs or even cause a fetus—origin adult disease.

This finding also reveals the possibility that maternal small-noncoding RNAs participate in fetal epigenetic regulation during pregnancy. Thus, the pathological status of the mother will result in an abnormal endogenous miRNA profile per se, which will also influence fetal health.

The article can be found at: Li et al. (2015) Small Non-coding RNAs Transfer Through Mammalian Placenta And Directly Regulate Fetal Gene Expression.


Source: Nanjing University; Photo: Thomas/Flickr/CC.
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