Sex Regulation By RNA Scissors

In persimmons, a battle of the sexes is waged using RNA scissors encoded on the Y chromosome that snip up female-determining genes.

AsianScientist (Nov. 10, 2014) – Persimmons are among the small club of plants with separate sexes—individual trees are either male or female. Now scientists at Kyoto University and the University of California, Davis have discovered how sex is determined in a species of persimmon, potentially opening up new possibilities in plant breeding. The work has been published in the journal Science.

Most plants have both male and female sex organs in the same individual. Some, like tomato, rice, beans and other cultivated species, cast pollen from male to female organs in the same flower. Others employ ingenious schemes to ensure that one individual pollinates the flower of another. Only about five percent of plant species have separate sexes, a condition called dioecy, or “two houses.”

“Think of it as nature’s best trick to ensure that reproduction involves two individuals, thus maximizing the mixing of genes. Persimmon, pistachio, wild grapevine, kiwi, hops, spinach and even marijuana are dioecious,” said Luca Comai, professor of plant biology at UC Davis and senior author on the paper.

In mammals, sex is determined by X and Y chromosomes: Males have an X and a Y; females have two X’s. A single gene on the Y is responsible for triggering the development of male traits. Most dioecious plants resemble the human system, with XY males and XX females. What gene may be responsible for determining plant sex has been a long-standing mystery.

Working on a family of persimmon trees (Diospyros lotus) established by Professor Ryutaro Tao at Kyoto University, researchers combed through the genomes of some of these trees looking for genes that were exclusive to males. They found an unusual gene they called OGI (Japanese for male tree). Unlike most genes, OGI does not encode a protein, they found. Instead, it codes for a very small piece of RNA that acts as “molecular scissors,” cutting down expression of another gene, called MeGI (Japanese for female tree).

In females, MeGI builds to high level and acts like a neutering agent, repressing pollen formation. In males, OGI prevents accumulation of MeGI. Regulation by RNA scissors can be fickle, and this may help explain why plants that are genetically one sex but functionally another can arise in dioecious species.

Discovery of the OGI-MeGI system in persimmon provides a comparison for parallel studies in other dioecious plant species, Prof. Comai said.

“Because separate sexes evolved independently many times in plants, we can effectively replay the evolutionary game and ask whether plants invent different solutions to the same problem or whether the same regulatory system is recruited over and over,” he said.

The findings may also have practical applications.

“Separate sexes are the most effective way to produce plant hybrids, and hybrids are key to agricultural productivity,” said Dr. Isabelle Henry, co-author of the study. “In the future, we may be able to breed dioecy into new species and facilitate hybrid production through exploitation of a natural system.”

The article can be found at: Akagi et al. (2014) A Y-chromosome–encoded Small RNA Acts as a Sex Determinant in Persimmons.


Source: University of California – Davis.
Disclaimer: This article does not necessarily reflect the views of AsianScientist or its staff.

Asian Scientist Magazine is an award-winning science and technology magazine that highlights R&D news stories from Asia to a global audience. The magazine is published by Singapore-headquartered Wildtype Media Group.

Related Stories from Asian Scientist